The Method Validation of the Quantification of Methamphetamine Using MPIC
Honors Capstone Project
1
Advisor(s)
Dr. Harold Schueler
Confirmation
1
Document Type
Paper
Location
Wishing Well
Start Date
16-4-2024 2:30 PM
End Date
16-4-2024 5:15 PM
Abstract
Isotopologues are nearly chemically identical to each other while still distinguishable on mass spectrometers due to the difference in atomic mass. Deuterium (²H or D) is an isotope of hydrogen with one additional neutron and is thus one atomic mass unit heavier than hydrogen. Isotopically labeled drugs are currently utilized as internal standards in the field of forensic toxicology. This is due to blood being a matrix that requires extraction prior to analysis and extraction processes do not have a uniform yield thus requiring a known concentration to compare the post-extraction concentration to. In Multipoint Internal Calibration (MPIC) different deuterated standards: methamphetamine D₅, D₈, D₉, D₁₁, and D₁₄ were added into the same sample at differing concentrations, 1000 ng/mL, 600 ng/mL, 300 ng/mL, 100 ng/mL, and 10 ng/mL, respectively. Six different concentrations of methamphetamine: 49.6 ng/mL, 120 ng/mL, 456 ng/mL, 552 ng/mL, 720 ng/mL, and 800 ng/mL were tested and their concentrations calculated. Each of these concentrations was sampled in triplicate for each of the 5 replicate extractions.
Recommended Citation
Gussenhoven, Katherine Adele, "The Method Validation of the Quantification of Methamphetamine Using MPIC" (2024). ONU Student Research Colloquium. 35.
https://digitalcommons.onu.edu/student_research_colloquium/2024/papers/35
Level of Access
Restricted to ONU Community
Restricted
Available to ONU community via local IP address and ONU login.
The Method Validation of the Quantification of Methamphetamine Using MPIC
Wishing Well
Isotopologues are nearly chemically identical to each other while still distinguishable on mass spectrometers due to the difference in atomic mass. Deuterium (²H or D) is an isotope of hydrogen with one additional neutron and is thus one atomic mass unit heavier than hydrogen. Isotopically labeled drugs are currently utilized as internal standards in the field of forensic toxicology. This is due to blood being a matrix that requires extraction prior to analysis and extraction processes do not have a uniform yield thus requiring a known concentration to compare the post-extraction concentration to. In Multipoint Internal Calibration (MPIC) different deuterated standards: methamphetamine D₅, D₈, D₉, D₁₁, and D₁₄ were added into the same sample at differing concentrations, 1000 ng/mL, 600 ng/mL, 300 ng/mL, 100 ng/mL, and 10 ng/mL, respectively. Six different concentrations of methamphetamine: 49.6 ng/mL, 120 ng/mL, 456 ng/mL, 552 ng/mL, 720 ng/mL, and 800 ng/mL were tested and their concentrations calculated. Each of these concentrations was sampled in triplicate for each of the 5 replicate extractions.