Advisor(s)

Phillip Zoladz

Confirmation

1

Document Type

Poster

Location

ONU McIntosh Center; Activities Room

Start Date

24-4-2026 10:00 AM

End Date

24-4-2026 10:50 AM

Abstract

Single treatments with psychedelics have demonstrated rapid, long-lasting antidepressant effects. However, psychedelics often produce unfavorable psychoactive effects that could worsen existing psychological distress. Thus, we compared the effects of psilocybin and the related tryptamine, norbaeocystin, on anxiety-like behavior in male and female rats. In Experiment 1, rats were treated with 1 mg/kg psilocybin (PSIL), 1 mg/kg norbaeocystin (NOR), or vehicle 30 min before a 5-min trial on an elevated plus maze (EPM) or in an open field test (OFT). On the EPM, PSIL led to decreased open arm time and fewer arm entries in males and females. NOR led to decreased open arm time in males, while having no impact on female behavior. PSIL led to decreased time in the center of the open field in males and decreased distance traveled in males and females. NOR led to decreased time in the center of the open field in males, while having no impact on female behavior. In Experiment 2, we assessed whether the anxiogenic effects of PSIL and NOR would persist for 24 hr. Rats were administered PSIL, NOR, or vehicle and tested on the EPM 24 hr later. Analyses revealed no effects of PSIL or NOR on EPM behavior. Our results indicate that PSIL exerts acute anxiogenic effects in both males and females, while NOR selectively increases anxiety-like behavior in males. These sex differences may be an important consideration in identifying which psychedelic-like compounds are most effective in treating psychological conditions in both sexes.

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Apr 24th, 10:00 AM Apr 24th, 10:50 AM

Psilocybin and the related tryptamine, norbaeocystin, exert acute anxiogenic effects that depend on sex

ONU McIntosh Center; Activities Room

Single treatments with psychedelics have demonstrated rapid, long-lasting antidepressant effects. However, psychedelics often produce unfavorable psychoactive effects that could worsen existing psychological distress. Thus, we compared the effects of psilocybin and the related tryptamine, norbaeocystin, on anxiety-like behavior in male and female rats. In Experiment 1, rats were treated with 1 mg/kg psilocybin (PSIL), 1 mg/kg norbaeocystin (NOR), or vehicle 30 min before a 5-min trial on an elevated plus maze (EPM) or in an open field test (OFT). On the EPM, PSIL led to decreased open arm time and fewer arm entries in males and females. NOR led to decreased open arm time in males, while having no impact on female behavior. PSIL led to decreased time in the center of the open field in males and decreased distance traveled in males and females. NOR led to decreased time in the center of the open field in males, while having no impact on female behavior. In Experiment 2, we assessed whether the anxiogenic effects of PSIL and NOR would persist for 24 hr. Rats were administered PSIL, NOR, or vehicle and tested on the EPM 24 hr later. Analyses revealed no effects of PSIL or NOR on EPM behavior. Our results indicate that PSIL exerts acute anxiogenic effects in both males and females, while NOR selectively increases anxiety-like behavior in males. These sex differences may be an important consideration in identifying which psychedelic-like compounds are most effective in treating psychological conditions in both sexes.