Mapping of the S6 Mutation in Drosophila Melanogaster
Advisor(s)
Dr. Jamie Siders
Confirmation
1
Document Type
Poster
Location
ONU McIntosh Center; Activities Room
Start Date
24-4-2026 10:00 AM
End Date
24-4-2026 10:50 AM
Abstract
The Fly-CURE is a multi-institutional research project that utilizes Drosophila melanogaster to investigate cell growth and development. The project leverages a Flp-FRT, EMS based genetic screen conducted in an AIF (Apoptosis Inducing Factor 1) mutant background to perturb noncanonical apoptotic pathways. The current study investigated the S6 EMS mutant, utilizing the Flp-FRT system to generate red (-/- ) and white (+/+) cells in a tissue specific manner in the eye. Control Ubi-RFP and AIF progeny displayed ratios of 70% red : 30% white and 15% red : 85% white, cells respectively. S6 experimental progeny displayed a ratio of 40% red : 60% white. In order to map the mutation, S6 flies were crossed with deficiency stocks. Bloomington stocks 9682 and 9266 failed to complement (FTC) with S6, however both of these stocks also FTC with a separate EMS mutant, 1.4JS, as such, the identified FTC region is likely a second mutation in AIF background strain. Five candidate genes were identified in this region based on this genetic mapping and whole genome sequencing: Hr39, L(2)k14505, CG8671, Mondo, and GR39a. Future work includes additional deficiency crosses and sequencing to identify the background AIF mutation, as well as additional deficiency crosses to identify S6 EMS mutation.
Recommended Citation
Cetera, Morgan L.; Heydinger, Lillian C.; Sowers, Madison K.; Bieser, Kayla L. Ph.D.; Evans, Cory J. Ph.D.; Hamill, Danielle R. Ph.D.; Kagey, Jacob R. Ph.D.; and Siders, Jamie L. Ph.D., "Mapping of the S6 Mutation in Drosophila Melanogaster" (2026). ONU Student Research Colloquium. 16.
https://digitalcommons.onu.edu/student_research_colloquium/2026/Posters/16
Open Access
Available to all.
Mapping of the S6 Mutation in Drosophila Melanogaster
ONU McIntosh Center; Activities Room
The Fly-CURE is a multi-institutional research project that utilizes Drosophila melanogaster to investigate cell growth and development. The project leverages a Flp-FRT, EMS based genetic screen conducted in an AIF (Apoptosis Inducing Factor 1) mutant background to perturb noncanonical apoptotic pathways. The current study investigated the S6 EMS mutant, utilizing the Flp-FRT system to generate red (-/- ) and white (+/+) cells in a tissue specific manner in the eye. Control Ubi-RFP and AIF progeny displayed ratios of 70% red : 30% white and 15% red : 85% white, cells respectively. S6 experimental progeny displayed a ratio of 40% red : 60% white. In order to map the mutation, S6 flies were crossed with deficiency stocks. Bloomington stocks 9682 and 9266 failed to complement (FTC) with S6, however both of these stocks also FTC with a separate EMS mutant, 1.4JS, as such, the identified FTC region is likely a second mutation in AIF background strain. Five candidate genes were identified in this region based on this genetic mapping and whole genome sequencing: Hr39, L(2)k14505, CG8671, Mondo, and GR39a. Future work includes additional deficiency crosses and sequencing to identify the background AIF mutation, as well as additional deficiency crosses to identify S6 EMS mutation.