Effects of pretreatment with the CB1 receptor agonist (WIN 55212) on cocaine-induced behavioral effects in male and female mice
Advisor(s)
Dr. Manoranjan S D'Souza
Confirmation
1
Document Type
Poster
Location
ONU McIntosh Center; Activities Room
Start Date
11-4-2025 12:00 PM
End Date
11-4-2025 12:50 PM
Abstract
Cocaine is an illicit substance that is widely abused in the Unites States. Over the last few years, cannabis and products utilizing cannabis are being legalized across the United States. This has led to their increasing use. Cannabis and products utilizing cannabis activate CB1 receptors in the brain, which can have several behavioral effects. More recently sex-dependent differences in the role of CB1 receptors have been reported. However, the effects of CB1 receptor activation in adult male and female mice on the rewarding and psychomotor effects of cocaine have not been systematically evaluated. This study aimed to address this gap in knowledge. Adult (12-18 weeks old) male and female mice (C57BL6) were used for the study. Cocaine-induced locomotor activity and rewarding effects were assessed at two doses of cocaine (5 and 15 mg/kg). The rewarding effects of cocaine were assessed using the conditioned place preference test (CPP). CB1 receptors were activated using CB1 agonist WIN 55212-2 (3 mg/kg). Data in this project is currently being collected and analyzed. We hypothesize that activation of CB1 receptors will have differential effects on cocaine-induced behavioral effects in male and female mice.
Recommended Citation
Lee, Jejun; Baughman, Cade; Lockridge, Demetrius; Seeley, Sarah; and D'Souza, Manoranjan S., "Effects of pretreatment with the CB1 receptor agonist (WIN 55212) on cocaine-induced behavioral effects in male and female mice" (2025). ONU Student Research Colloquium. 72.
https://digitalcommons.onu.edu/student_research_colloquium/2025/Posters/72
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Effects of pretreatment with the CB1 receptor agonist (WIN 55212) on cocaine-induced behavioral effects in male and female mice
ONU McIntosh Center; Activities Room
Cocaine is an illicit substance that is widely abused in the Unites States. Over the last few years, cannabis and products utilizing cannabis are being legalized across the United States. This has led to their increasing use. Cannabis and products utilizing cannabis activate CB1 receptors in the brain, which can have several behavioral effects. More recently sex-dependent differences in the role of CB1 receptors have been reported. However, the effects of CB1 receptor activation in adult male and female mice on the rewarding and psychomotor effects of cocaine have not been systematically evaluated. This study aimed to address this gap in knowledge. Adult (12-18 weeks old) male and female mice (C57BL6) were used for the study. Cocaine-induced locomotor activity and rewarding effects were assessed at two doses of cocaine (5 and 15 mg/kg). The rewarding effects of cocaine were assessed using the conditioned place preference test (CPP). CB1 receptors were activated using CB1 agonist WIN 55212-2 (3 mg/kg). Data in this project is currently being collected and analyzed. We hypothesize that activation of CB1 receptors will have differential effects on cocaine-induced behavioral effects in male and female mice.