Preliminary Analysis of the Antimicrobial and Anti-inflammatory Activity of Lobelia Species Medicinally Used by Native Americans
Advisor(s)
Dr. Jill Bennett-Toomey
Dr. Lisa Walden
Dr. Linda Young
Confirmation
1
Document Type
Poster
Location
McIntosh Activities Room
Start Date
19-4-2024 10:00 AM
End Date
19-4-2024 10:50 AM
Abstract
Historical use of Lobelia species suggests antimicrobial and anti-inflammatory activity. We screened the following constituents for antimicrobial activity: perilla ketone, δ-carene, myrcene, isophytol, allyl nonanoate, limonene, camphorquinone and lobelin hydrochloride. These constituents were applied at ~ 10 µg/6mm filter paper dot for Kirby-Bauer assay against Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Zones of inhibition were observed against S. pneumoniae for perilla ketone (21 mm), allyl nonanoate (14.5 mm) and myrcene (8 mm). Synergy studies with these 3 constituents are on-going. Ethanolic extracts of Lobelia species cardinalis, inflata and siphilitica were applied at 100 µg plant material/dot for Kirby-Bauer assays against Streptococcus pyogenes, carbapenem-resistant Klebsiella pneumoniae, vancomycin-resistant Enterococcus faecalis and the previously screened microbes. S. pneumoniae again developed zones of inhibition: cardinalis (10.3 mm), inflata, (9.3 mm) and siphilitica (9.7 mm), showing moderate antibiotic efficacy with all species. Anti-inflammatory activity was examined by immunocytochemistry. Lobelia spp. extracts were used to treat mouse granulosa cells at 30 minutes and 1 hour following LPS-induced inflammation. Cardinalis showed minimal suppression of inflammation. Siphilitica was shown to suppress inflammation within the 30-minute treatment, followed by Inflata suppression within the 1 hour treatment.
Recommended Citation
Converse-Olson, Makkenzi; Bearer, Alex; Bitters, Abigail; Falke, Luke; and Hampton, Faith, "Preliminary Analysis of the Antimicrobial and Anti-inflammatory Activity of Lobelia Species Medicinally Used by Native Americans" (2024). ONU Student Research Colloquium. 27.
https://digitalcommons.onu.edu/student_research_colloquium/2024/Posters/27
Level of Access
Open Access
Open Access
Available to all.
Preliminary Analysis of the Antimicrobial and Anti-inflammatory Activity of Lobelia Species Medicinally Used by Native Americans
McIntosh Activities Room
Historical use of Lobelia species suggests antimicrobial and anti-inflammatory activity. We screened the following constituents for antimicrobial activity: perilla ketone, δ-carene, myrcene, isophytol, allyl nonanoate, limonene, camphorquinone and lobelin hydrochloride. These constituents were applied at ~ 10 µg/6mm filter paper dot for Kirby-Bauer assay against Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Zones of inhibition were observed against S. pneumoniae for perilla ketone (21 mm), allyl nonanoate (14.5 mm) and myrcene (8 mm). Synergy studies with these 3 constituents are on-going. Ethanolic extracts of Lobelia species cardinalis, inflata and siphilitica were applied at 100 µg plant material/dot for Kirby-Bauer assays against Streptococcus pyogenes, carbapenem-resistant Klebsiella pneumoniae, vancomycin-resistant Enterococcus faecalis and the previously screened microbes. S. pneumoniae again developed zones of inhibition: cardinalis (10.3 mm), inflata, (9.3 mm) and siphilitica (9.7 mm), showing moderate antibiotic efficacy with all species. Anti-inflammatory activity was examined by immunocytochemistry. Lobelia spp. extracts were used to treat mouse granulosa cells at 30 minutes and 1 hour following LPS-induced inflammation. Cardinalis showed minimal suppression of inflammation. Siphilitica was shown to suppress inflammation within the 30-minute treatment, followed by Inflata suppression within the 1 hour treatment.