MONARDA SUPPRESSES LPS-INDUCED INFLAMMATION IN MOUSE GRANULOSA CELLS.
Advisor(s)
Jill Bennett-Toomey
Confirmation
1
Document Type
Poster
Location
ONU McIntosh Center; McIntosh Activities Room
Start Date
21-4-2023 10:00 AM
End Date
21-4-2023 10:50 AM
Abstract
Ethnopharmacology is the study of the use of native plants by indigenous people for medicinal purposes. The wide range of healing capabilities of plants, including anti-inflammatory, antibiotic, and anti-cancer action, is based on their chemical constituents. Plants in the genus Monarda, which have high levels of monoterpenes, were used by Native Americans to treat sore throats, intestinal issues, inflammation, and other complaints. Previous studies in this lab demonstrated that extracts of Monarda spp. administered prior to proinflammatory lipopolysaccharide (LPS) reduces development of inflammation. This study focused on the use of M. fistulosa, M.citriodora, M. didyma, and M. punctata as treatment for existing inflammation. To carry out this experiment Monarda spp. extracts were used to treat mouse granulosa cells which expressed LPS induced inflammation. Inflammation was assessed visually using immunocytochemistry and quantitatively via interleukin-6 assays. Monarda extracts are significantly more effective in treating than in preventing LPS-induced inflammation (p =0.03, Student’s t-test). Future research includes a time course study to determine how quickly the Monarda extracts suppress the IL6 inflammatory pathway as well as measuring levels of other inflammatory mediators and responses to other proinflammatory compounds.
Recommended Citation
Converse-Olson, Makke, "MONARDA SUPPRESSES LPS-INDUCED INFLAMMATION IN MOUSE GRANULOSA CELLS." (2023). ONU Student Research Colloquium. 27.
https://digitalcommons.onu.edu/student_research_colloquium/2023/posters/27
Open Access
Available to all.
MONARDA SUPPRESSES LPS-INDUCED INFLAMMATION IN MOUSE GRANULOSA CELLS.
ONU McIntosh Center; McIntosh Activities Room
Ethnopharmacology is the study of the use of native plants by indigenous people for medicinal purposes. The wide range of healing capabilities of plants, including anti-inflammatory, antibiotic, and anti-cancer action, is based on their chemical constituents. Plants in the genus Monarda, which have high levels of monoterpenes, were used by Native Americans to treat sore throats, intestinal issues, inflammation, and other complaints. Previous studies in this lab demonstrated that extracts of Monarda spp. administered prior to proinflammatory lipopolysaccharide (LPS) reduces development of inflammation. This study focused on the use of M. fistulosa, M.citriodora, M. didyma, and M. punctata as treatment for existing inflammation. To carry out this experiment Monarda spp. extracts were used to treat mouse granulosa cells which expressed LPS induced inflammation. Inflammation was assessed visually using immunocytochemistry and quantitatively via interleukin-6 assays. Monarda extracts are significantly more effective in treating than in preventing LPS-induced inflammation (p =0.03, Student’s t-test). Future research includes a time course study to determine how quickly the Monarda extracts suppress the IL6 inflammatory pathway as well as measuring levels of other inflammatory mediators and responses to other proinflammatory compounds.