Sponsor
Dennis J. De Luca, Ph. D
Ohio Northern University
Biological and Allied Health Sciences
d-deluca@onu.edu
Advisor(s)
Heather E. McKiernan, Ph. D
The Center for Forensic Science Research and Education
heather.mckiernan@cfsre.org
Catherine O. Brown, MSFS
The Center for Forensic Science Research and Education
catherine.brown@cfsre.org
Document Type
Poster
Start Date
24-4-2020 9:00 AM
Abstract
In the United States a sexual assault is committed every 92 seconds. Due to limited funding, time, and other resources this has caused the US to accumulate a large sexual assault kit (SAK) backlog. In order to combat this backlog advanced methods for complete SAK testing are readily being developed. This study was done to compare a validated in-house method for co-extraction of DNA and proteins developed at the Center for Forensic Science Research and Education to previously validated methods developed by Kranes et. al. and the commercially available Qiagen AllPrep™ DNA/RNA/Protein Mini Kit. The three sample fractionation protocols were compared according to the following criteria: (1) peak area intensity observed via UPLC-MS/MS analysis for target protein biomarkers; (2) DNA quantification values; (3) overall quality of genetic profile obtained; (4) consistency among preparation replicates; and (5) speed/cost and ease of workflow. These data demonstrate that sufficient DNA and protein can be obtained from simulated sexual assault samples to allow for the simultaneous forensic analysis of each fraction, eliminating the need for forensic analysts to prioritize one type of testing over the other.
Recommended Citation
Peters, Morgan S., "Front-End Fractionation of DNA and Proteins for the Simultaneous Genetic and Serological Analysis of Sexual Assault Case Samples" (2020). ONU Student Research Colloquium. 25.
https://digitalcommons.onu.edu/student_research_colloquium/2020/posters/25
Restricted
Available to ONU community via local IP address and ONU login.
Front-End Fractionation of DNA and Proteins for the Simultaneous Genetic and Serological Analysis of Sexual Assault Case Samples
In the United States a sexual assault is committed every 92 seconds. Due to limited funding, time, and other resources this has caused the US to accumulate a large sexual assault kit (SAK) backlog. In order to combat this backlog advanced methods for complete SAK testing are readily being developed. This study was done to compare a validated in-house method for co-extraction of DNA and proteins developed at the Center for Forensic Science Research and Education to previously validated methods developed by Kranes et. al. and the commercially available Qiagen AllPrep™ DNA/RNA/Protein Mini Kit. The three sample fractionation protocols were compared according to the following criteria: (1) peak area intensity observed via UPLC-MS/MS analysis for target protein biomarkers; (2) DNA quantification values; (3) overall quality of genetic profile obtained; (4) consistency among preparation replicates; and (5) speed/cost and ease of workflow. These data demonstrate that sufficient DNA and protein can be obtained from simulated sexual assault samples to allow for the simultaneous forensic analysis of each fraction, eliminating the need for forensic analysts to prioritize one type of testing over the other.