Advisor(s)

Jamie Siders, PhD
Ohio Northern University
Biological Sciences, Science, Technology, and Mathematics
j-siders@onu.edu

Kenneth Oswald, PhD
Ohio Northern University
Environmental & Field Biology, Science, Technology, and Mathematics
k-oswald@onu.edu

Document Type

Poster

Location

ONU McIntosh Center; Activities Room

Start Date

22-4-2022 10:00 AM

End Date

22-4-2022 11:00 AM

Abstract

A singular population of tonguetied minnow (Exoglossum laurae) inhabits a ~50-km segment of the Mad River of western Ohio. Tonguetied minnow is Endangered in Ohio as a consequence of its small geographic range and low abundance. For this study, the polymerase chain reaction (PCR) was used amplify five genetic loci from 35 tonguetied minnow captured from the Mad River. Loci included two mitochondrial genes, the NADH-dehydrogenase 2 (ND2) and the control region (D-loop). From the nuclear genome, myosin heavy chain 6 (myh6) and β-actin (β-act) introns were amplified, as well as a portion of the major histocompatibility (Mhc-IIB) locus. PCR amplifications were sequenced using the Sanger method, and these sequence data provided estimates of population genetic diversity. Results showed no genetic diversity (h = 0) at D-loop, myh6, and β-act. The ND2 locus showed evidence for low-moderate levels of genetic diversity. The Mhc-IIB showed slight variation, although it was not possible to obtain an estimate for genetic variation at this locus due to an insertion/deletion mutation that left sequences from many individuals unreadable. The Mhc-IIB PCR products from these individuals are currently being cloned so that genetic diversity can be estimated at this gene. Future applications of these results will involve determining the relative contributions of genetic drift versus natural selection on tonguetied minnow as well as to assist in the conservation and management of this imperiled population.

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Apr 22nd, 10:00 AM Apr 22nd, 11:00 AM

Genetic Variation in the Ohio Population of Tonguetied Minnow (Exoglossum laurae)

ONU McIntosh Center; Activities Room

A singular population of tonguetied minnow (Exoglossum laurae) inhabits a ~50-km segment of the Mad River of western Ohio. Tonguetied minnow is Endangered in Ohio as a consequence of its small geographic range and low abundance. For this study, the polymerase chain reaction (PCR) was used amplify five genetic loci from 35 tonguetied minnow captured from the Mad River. Loci included two mitochondrial genes, the NADH-dehydrogenase 2 (ND2) and the control region (D-loop). From the nuclear genome, myosin heavy chain 6 (myh6) and β-actin (β-act) introns were amplified, as well as a portion of the major histocompatibility (Mhc-IIB) locus. PCR amplifications were sequenced using the Sanger method, and these sequence data provided estimates of population genetic diversity. Results showed no genetic diversity (h = 0) at D-loop, myh6, and β-act. The ND2 locus showed evidence for low-moderate levels of genetic diversity. The Mhc-IIB showed slight variation, although it was not possible to obtain an estimate for genetic variation at this locus due to an insertion/deletion mutation that left sequences from many individuals unreadable. The Mhc-IIB PCR products from these individuals are currently being cloned so that genetic diversity can be estimated at this gene. Future applications of these results will involve determining the relative contributions of genetic drift versus natural selection on tonguetied minnow as well as to assist in the conservation and management of this imperiled population.