Advisor(s)

Kenneth J. Oswald, PhD
Ohio Northern University
Environmental & Field Biology, Science, Technology, and Mathematics
k-oswald@onu.edu

Document Type

Poster

Start Date

23-4-2021 9:00 AM

Description

Tonguetied minnow (Exoglossum laurae) is a freshwater cyprinid that has a single extant population in the Mad River of Ohio. Due to its restricted range, tonguetied minnow is endangered in Ohio. A total of 35 tonguetied minnows were collected from the Mad River. Caudal fin clips were taken from each individual and genomic DNA was extracted from each clip. This DNA was then used as template for polymerase chain reaction (PCR). Successful PCR amplifications have been obtained for two mitochondrial genes (ND2 and D-loop), two nuclear introns (MYH and β-actin), and two nuclear coding genes (HSP70 and MHCIIβ). These PCR products are now being sequenced to characterize genetic diversity within this population. These data can eventually be used to help conserve this imperiled population.

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Apr 23rd, 9:00 AM

Genetic Data for Ohio's Endangered Population of Tonguetied Minnow (Exoglossum laurae)

Tonguetied minnow (Exoglossum laurae) is a freshwater cyprinid that has a single extant population in the Mad River of Ohio. Due to its restricted range, tonguetied minnow is endangered in Ohio. A total of 35 tonguetied minnows were collected from the Mad River. Caudal fin clips were taken from each individual and genomic DNA was extracted from each clip. This DNA was then used as template for polymerase chain reaction (PCR). Successful PCR amplifications have been obtained for two mitochondrial genes (ND2 and D-loop), two nuclear introns (MYH and β-actin), and two nuclear coding genes (HSP70 and MHCIIβ). These PCR products are now being sequenced to characterize genetic diversity within this population. These data can eventually be used to help conserve this imperiled population.