Paper/Poster Title
Genetic Data for Ohio's Endangered Population of Tonguetied Minnow (Exoglossum laurae)
Sponsor
Kenneth J. Oswald, PhD
Ohio Northern University
Environmental & Field Biology, Science, Technology, and Mathematics
k-oswald@onu.edu
Advisor(s)
Kenneth J. Oswald, PhD
Ohio Northern University
Environmental & Field Biology, Science, Technology, and Mathematics
k-oswald@onu.edu
Document Type
Poster
Start Date
23-4-2021 9:00 AM
Abstract
Tonguetied minnow (Exoglossum laurae) is a freshwater cyprinid that has a single extant population in the Mad River of Ohio. Due to its restricted range, tonguetied minnow is endangered in Ohio. A total of 35 tonguetied minnows were collected from the Mad River. Caudal fin clips were taken from each individual and genomic DNA was extracted from each clip. This DNA was then used as template for polymerase chain reaction (PCR). Successful PCR amplifications have been obtained for two mitochondrial genes (ND2 and D-loop), two nuclear introns (MYH and β-actin), and two nuclear coding genes (HSP70 and MHCIIβ). These PCR products are now being sequenced to characterize genetic diversity within this population. These data can eventually be used to help conserve this imperiled population.
Recommended Citation
Armstrong, Sarah; Gill, Hannah; Gremling, Emily E.; Steiner, Tad; and Williams, Madeline, "Genetic Data for Ohio's Endangered Population of Tonguetied Minnow (Exoglossum laurae)" (2021). ONU Student Research Colloquium. 2.
https://digitalcommons.onu.edu/student_research_colloquium/2021/posters/2
Restricted
Available to ONU community via local IP address and ONU login.
Genetic Data for Ohio's Endangered Population of Tonguetied Minnow (Exoglossum laurae)
Tonguetied minnow (Exoglossum laurae) is a freshwater cyprinid that has a single extant population in the Mad River of Ohio. Due to its restricted range, tonguetied minnow is endangered in Ohio. A total of 35 tonguetied minnows were collected from the Mad River. Caudal fin clips were taken from each individual and genomic DNA was extracted from each clip. This DNA was then used as template for polymerase chain reaction (PCR). Successful PCR amplifications have been obtained for two mitochondrial genes (ND2 and D-loop), two nuclear introns (MYH and β-actin), and two nuclear coding genes (HSP70 and MHCIIβ). These PCR products are now being sequenced to characterize genetic diversity within this population. These data can eventually be used to help conserve this imperiled population.