Document Type

Poster

Start Date

24-4-2020 9:00 AM

Description

Tonguetied minnow (Exoglossum laurae) is a cold-water specialist that is a rare surviving remnant of the Pleistocene. Tonguetied minnow is Endangered in Ohio where a single population is confined to a ~60km segment of the Mad River. Preservation of tonguetied minnow is of particular paleontological and biotechnological interest due to the species' highly restricted and fragmented zoogeography as well as its evolutionary adaptation to cold water. Thirty-five tonguetied minnow were collected from the Mad River, and genomic DNA (gDNA) was extracted from fin clips and used as template in the polymerase chain reaction (PCR). Five genetic markers have been developed, including the mitochondrial control region (D-loop) and NADH dehydrogenase 2 (nd2) loci as well as the nuclear-encoded β-actin (β-act), heat shock protein 70 (hsp70), and major histocompatibility class IIB (MHCIIB) loci. DNA sequencing of PCR products has revealed low genetic variation (h ≈ 0) for nd2 and β-act. Comparisons of variation among loci and among individuals will yield insights into evolutionary processes such as genetic drift and natural selection within this population. Genetic data will be used to inform and promote conservationefforts for this highly imperiled population, including subsequent comparisons to whole genome sequencing.

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Apr 24th, 9:00 AM

Genetic Diversity in Ohio's Endangered Population of Tonguetied Minnow (Exoglossum laurae)

Tonguetied minnow (Exoglossum laurae) is a cold-water specialist that is a rare surviving remnant of the Pleistocene. Tonguetied minnow is Endangered in Ohio where a single population is confined to a ~60km segment of the Mad River. Preservation of tonguetied minnow is of particular paleontological and biotechnological interest due to the species' highly restricted and fragmented zoogeography as well as its evolutionary adaptation to cold water. Thirty-five tonguetied minnow were collected from the Mad River, and genomic DNA (gDNA) was extracted from fin clips and used as template in the polymerase chain reaction (PCR). Five genetic markers have been developed, including the mitochondrial control region (D-loop) and NADH dehydrogenase 2 (nd2) loci as well as the nuclear-encoded β-actin (β-act), heat shock protein 70 (hsp70), and major histocompatibility class IIB (MHCIIB) loci. DNA sequencing of PCR products has revealed low genetic variation (h ≈ 0) for nd2 and β-act. Comparisons of variation among loci and among individuals will yield insights into evolutionary processes such as genetic drift and natural selection within this population. Genetic data will be used to inform and promote conservationefforts for this highly imperiled population, including subsequent comparisons to whole genome sequencing.