Advisor(s)
Dr. Manoranjan S D'Souza: m-dsouza@onu.edu
Confirmation
1
Document Type
Poster
Location
McIntosh Activities Room
Start Date
19-4-2024 10:00 AM
End Date
19-4-2024 10:50 AM
Abstract
The overall objective of the study was to assess the acute behavioral effects of currently used antidepressants and anxiolytics in male and female mice lacking regulator of G protein-signaling (RGS) proteins 2 and 4 and their wild-type counterparts. RGS 2 and 4 proteins negatively modulate signaling pathways of G protein-coupled receptors (GPCRs), which play an important role in mediating the effects of monoamine neurotransmitters such as dopamine, norepinephrine, and serotonin. These neurotransmitters in turn play an important role in the action of antidepressant and anxiolytic medications. The study was undertaken because no studies till date have systematically assessed the behavioral effects of anxiolytics and antidepressants in both male and female mice lacking RGS2 and RGS4 proteins. Anxiety-like behaviors were assessed using the elevated plus maze (EPM) and antidepressant-like effects were assessed using the tail suspension test (TST). The anxiolytic and antidepressant drugs evaluated in this study include those that primarily target monoamine neurotransmission such as fluoxetine (30 mg/kg), desipramine (30 mg/kg), and buspirone (3 mg/kg). Data analyzed till date suggests that RGS2, but not RGS4, sex-dependently influences the antidepressant-like effect of desipramine and fluoxetine after acute administration. Moreover, the data suggests that RGS4, but not RGS2, sex-dependently influences the effects of buspirone and fluoxetine on anxiety-like behavior. Further doses of the above mentioned drugs are currently being evaluated. Data obtained from these studies will help in understanding the anxiolytic and antidepressant effects of drugs in humans carrying polymorphisms of the RGS2 and RGS4 genes.
Key Words: Behavior, Sex, RGS2, RGS4, G proteins, Neurotransmitters, Depression, Anxiety, Antidepressants, Anxiolytics
Recommended Citation
Matsui, Hiroyoshi; Seeley, Sarah; and D'Souza, Manoranjan S., "Differential behavioral responses in male and female mice lacking either RGS2 or RGS4 proteins after acute administration of antidepressants and anxiolytics" (2024). ONU Student Research Colloquium. 39.
https://digitalcommons.onu.edu/student_research_colloquium/2024/Posters/39
Level of Access
Open Access
Open Access
Available to all.
Included in
Behavioral Neurobiology Commons, Genetics Commons, Laboratory and Basic Science Research Commons, Neurosciences Commons, Pharmacology Commons, Substance Abuse and Addiction Commons
Differential behavioral responses in male and female mice lacking either RGS2 or RGS4 proteins after acute administration of antidepressants and anxiolytics
McIntosh Activities Room
The overall objective of the study was to assess the acute behavioral effects of currently used antidepressants and anxiolytics in male and female mice lacking regulator of G protein-signaling (RGS) proteins 2 and 4 and their wild-type counterparts. RGS 2 and 4 proteins negatively modulate signaling pathways of G protein-coupled receptors (GPCRs), which play an important role in mediating the effects of monoamine neurotransmitters such as dopamine, norepinephrine, and serotonin. These neurotransmitters in turn play an important role in the action of antidepressant and anxiolytic medications. The study was undertaken because no studies till date have systematically assessed the behavioral effects of anxiolytics and antidepressants in both male and female mice lacking RGS2 and RGS4 proteins. Anxiety-like behaviors were assessed using the elevated plus maze (EPM) and antidepressant-like effects were assessed using the tail suspension test (TST). The anxiolytic and antidepressant drugs evaluated in this study include those that primarily target monoamine neurotransmission such as fluoxetine (30 mg/kg), desipramine (30 mg/kg), and buspirone (3 mg/kg). Data analyzed till date suggests that RGS2, but not RGS4, sex-dependently influences the antidepressant-like effect of desipramine and fluoxetine after acute administration. Moreover, the data suggests that RGS4, but not RGS2, sex-dependently influences the effects of buspirone and fluoxetine on anxiety-like behavior. Further doses of the above mentioned drugs are currently being evaluated. Data obtained from these studies will help in understanding the anxiolytic and antidepressant effects of drugs in humans carrying polymorphisms of the RGS2 and RGS4 genes.
Key Words: Behavior, Sex, RGS2, RGS4, G proteins, Neurotransmitters, Depression, Anxiety, Antidepressants, Anxiolytics